In the case of tyrosine, nitration in the 3-position of the aromatic ring, producing 3-nitrotyrosine

Other protein-counting method, the method has been to count protein nitration. In this method, aromatic amino acid residue with nitric acid, and products of nitriding at 358 nm absorb. In the case of tyrosine, nitration in the 3-position of the aromatic ring, producing 3-nitrotyrosine. This method has been compared with the Bradford assay with BSA and the Smith standard, and almost the same results are obtained. The detection limit is 5 mg test and disorders known sailor X-100 and